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一种由病原体诱导的假定NAC转录因子调解大麦的叶子生抗性

Chunhong Chen¹, Matthias Jost¹, Megan A Outram¹

¹CSIRO Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1700, Canberra, ACT, 2601, Australia.

Nature communications

|September 6, 2023

概括

研究人员分离了Rph7基因,这是一种新的大麦叶抗基因. 这一发现提高了对植物防御机制的理解,并为作物疾病控制提供了新的策略.

科学领域:

植物病理学植物病理学
分子遗传学分子遗传学
农作物科学农作物科学

背景情况:

由Puccinia hordei引起的叶是大麦生产的主要威胁.
在大麦中的Rph7抵抗位点表现出显著的序列和哈普洛型分歧,表明了独特的进化路径.

研究的目的:

隔离和鉴定Rph7基因,该基因负责对大麦叶生的抗性.
阐明Rph7抗性蛋白的功能和结构特征.

主要方法:

使用精细映射和RNA测序 (RNA-Seq) 来识别Rph7基因.
突变分析和转基因补充用于基因验证.
用AlphaFold2蛋白模型进行结构分析.

主要成果:

通过功能测试成功分离并确认了Rph7基因.
Rph7编码了一种新型的,病原体诱导的耐药性蛋白质,具有NAC转录因子域和指BED域.
基因表达分析表明Rph7在激活和调节基底防御反应中的作用.

结论:

Rph7基因代表了Triticeae中一种独特的植物疾病抵抗基因类.
了解Rph7为植物免疫的多样化提供了洞察力.
Rph7基因提供了在作物中增强抗病能力的潜力.

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