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在共价有机框架上的酶固定支持支持

Qianqian Zhu^1,2, Yunlong Zheng^1,3, Zhenjie Zhang^4,5

¹State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, P.R. China.

Nature protocols

|September 6, 2023

概括

使用共价有机框架 (COFs) 的酶固定克服了工业酶使用的局限性. 直接固定方法可以创建稳定的酶@COF生物复合材料,其功能和可重复使用性得到改善.

科学领域:

材料科学材料科学材料科学
生物技术是生物技术.
化学工程是化学工程的重要组成部分.

背景情况:

酶是有效的自然催化剂,但由于敏感性和可重复使用性问题,它们面临着工业限制.
在固体支物中的酶固定,如共价有机框架 (COF),提供了一种提高稳定性和恢复的解决方案.
在COF中,合成后固定具有诸如酶浸出和孔隙阻塞等挑战.

研究的目的:

描述用于制造酶@COF生物复合材料的直接固定方法.
为了使各种大小的酶能够进行量身定制的结合,减少浸出和改善质量传输.
在恶劣条件下在COF合成期间提出酶保护策略.

主要方法:

通过在酶周围建立COF来直接固定.
使用可拆卸的金属有机框架 (MOF) 进行激烈COF合成的酶保护.
在温和条件下的现场直接固定,用于酶和COF单体组合.
酶@COF-42-B/43-B囊和脂酶@NKCOF-98/99.99的制造. 这是一个非常重要的过程.

主要成果:

成功制造具有编程结构和功能的酶@COF生物复合材料.
与后合成方法相比,证明减少了酶出和增强了质量运输.
实现了酶的定制整合,包括催化酶,葡萄糖氧化酶和脂酶.
对COF和enzyme@COF材料进行了表征分析.

结论:

直接固定方法提供了一个强大的策略,用于创建稳定和功能性的酶@COF生物复合材料.
这些方法解决了工业酶应用的关键局限性,提高了可重复使用性和性能.
描述的协议提供了在多孔材料中进行酶封装的多功能方法.

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