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Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
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相关实验视频

Updated: Jul 17, 2025

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
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基于N-异环碳素的黄金蚀刻剂

Robert B Chevalier1, Justin Pantano1, Matthew K Kiesewetter1

  • 1Department of Chemistry, University of Rhode Island, 140 Flagg Road, Kingston, RI, 02881, USA.

Beilstein journal of nanotechnology
|September 7, 2023
PubMed
概括
此摘要是机器生成的。

N-异环碳 (NHCs) 为黄金表面功能化提供了替代硫醇的替代品. 溶剂选择至关重要,因为一些NHC解决方案可以蚀刻金膜,而另一些则不能.

关键词:
其他N-异环碳化合物.黄金蚀刻 黄金蚀刻微型制造是指微型制造.自组装单层 (SAM) 自组装单层 (SAM)薄膜是一种薄膜.

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科学领域:

  • 表面化学 表面化学
  • 材料科学是一种材料科学.
  • 纳米技术 纳米技术

背景情况:

  • 越来越多的N-异环碳化合物 (NHCs) 正在被探索,作为替代醇的替代品,用于在黄金表面创建自组装单层 (SAM).
  • 与传统的基于醇的系统相比,基于NHC的SAM在稳定性和功能方面具有潜在的优势.

研究的目的:

  • 研究各种N-异环碳素 (NHC) 前体和溶剂对金色薄膜的影响.
  • 用NHC化学评估不必要的蚀刻和受控的表面修饰的可能性.

主要方法:

  • 四种不同的NHC物种的合成和应用:1,3-二 bisopropyl-5-nitrobenzimidazolium化物,1,3-二 bisopropyl-5-nitrobenzimidazolium碳酸, bis ((1,3-二 bisopropyl-5-nitrobenzimidazolium) 金 ((I) 化物,以及1,3-二 bisopropyl-5-nitrobenzimidazole-2-ylidene.
  • 暴露金涂玻璃幻灯片这些NHC物种在不同溶剂 (四二,二甲,烯) 中的溶液.
  • 用扫描电子显微镜 (SEM) 和能量分散式X射线光谱 (EDS) 分析金膜完整性的元素成像.

主要成果:

  • 在四二甲和二甲中溶解的1,3-二-5-二甲胺酸和1,3-二-5-二甲胺碳酸导致明显的黄金损失 (蚀刻).
  • 所有测试的NHC物种的托卢溶液并没有导致金膜明显溶解或蚀刻.
  • SEM和EDS分析证实了NHC物种和溶剂对黄金膜形态和组成的差异性影响.

结论:

  • 溶剂的选择是基于NHC的黄金表面功能化的关键参数,影响黄金膜的稳定性.
  • 某些NHC前体和溶剂存在意外黄金蚀刻的风险,需要对SAM形成进行仔细选择.
  • 这项研究表明,通过明智地选择NHC物种和溶剂,可以进行受控的黄金蚀刻,从而为故意的表面改造打开了道路.