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评估静态混合器作为一种新的微流体方法,用于脂质体配方

Aoba Ota¹, Ayaka Mochizuki¹, Keitaro Sou²

¹Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

Frontiers in bioengineering and biotechnology

|September 7, 2023

概括

静态混合器 (SM) 为脂质体配方提供了一个用户友好和有效的替代方案,可以很好地控制颗粒大小分布. 虽然与微流体设备相比,观察到封装效率略低,但SM在实际脂质体生产方面表现有前途.

科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
制药科学制药科学

背景情况:

传统的脂质体配方批量方法在可扩展性和精确控制实验条件方面面临挑战.
微流体设备提供了潜在的改进,但普遍采用的实用方法仍然难以捉摸.

研究的目的:

评估静态混合器 (SM) 作为微流体替代品的有效性,用于脂质体配方.
在关键配方参数方面,将SM性能与分阶鱼骨微混器 (SHM) 进行比较.

主要方法:

使用静态混合器 (SM) 和分层鱼骨微混合器 (SHM) 制备脂质体.
评估的关键参数包括封装效率,负载能力,层状度,颗粒大小和多分散度指数 (PDI).
还评估了用户友好性和易于清洗.

主要成果:

增加的初始脂质度导致SM和SHM的颗粒大小更大,SM产生更大的整体大小.
静态混合器 (SM) 的PDI显著较小,表明对颗粒大小分布的控制优越.
封装效率在SM中略低,但随着脂质度的增加而增加,尽管由于增加了片状度,低于理论值.

结论:

静态混合器 (SM) 在易用性方面提供了显著的优势,特别是在设备清洁和实验准备方面.
SMs可以产生均分布的脂质体,并为现有方法提供了切实可行的替代方案.
对SM混合元件的进一步修改可能会增强其用于先进脂质体配方的潜力.

关键词:

封装的封装方式脂质体组是什么? 脂质体组是什么?微流体学在微流体学方面纳米粒子是一种纳米粒子.摇摆着的鱼骨微混合器静态混合器静态混合器

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