ARID4B的丧失导致小鼠胚胎干细胞中的STAT1依赖干扰素通路被激活,并且在中皮/内皮分化过程中被激活
1Department of Biological Sciences, Middle East Technical University, Faculty of Arts and Sciences, Ankara, Turkey.
Journal of the Turkish German Gynecological Association
|September 7, 2023
概括
在小鼠胚胎干细胞分化过程中,ARID4B抑制干扰素基因. 它的缺失导致干扰素基因激活和染色质标记的改变,影响分化.
科学领域:
- 干细胞生物学 干细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因调节 基因调节
背景情况:
- 小鼠胚胎干细胞 (mESC) 的分化需要精确控制基因表达.
- ARID4B是一种染色质因子,对中皮和内皮分化至关重要.
- ARID4B 在基因素脱乙酶复合体内起作用,抑制基因.
研究的目的:
- 研究ARID4B在mESC分化的机制.
- 识别在ARID4B缺席的情况下升级调节的基因和通路.
- 阐明ARID4B在分化过程中的染色质修饰中的作用.
主要方法:
- 野生类型和干旱4bΔ mESCs的比较转录基因分析.
- 定量PCR,用于基因和蛋白质表达的西方涂抹.
- 对H3K4me3,H3K27me3和H3K27Ac.Ac.进行染色体免疫沉测序 (ChIP-seq).
主要成果:
- 与干扰素相关的基因在arid4bΔ mESCs中显著上调.
- 在arid4bΔ mESC中观察到酸化STAT1的水平增加.
- 由于ARID4B缺乏,干扰素基因调节区域的H3K4me3和H3K27me3/H3K27Ac水平发生了变化.
结论:
- 在mESC和分化过程中,ARID4B抑制了与干扰素相关的基因表达.
- 这种抑制是由调节色素标记,特别是H3K4me3.3.介导的.
- 通过ARID4B的调节对成功的mESC差异化具有潜在的重要意义.
相关概念视频
The JAK-STAT Signaling Pathway
9.0K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.0K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K


