在小鼠和仓鼠中对SARS-CoV-2 BA.5.5和BQ.1.1 Omicron变体的表征
James Brett Case1, Suzanne M Scheaffer1, Tamarand L Darling1
1Department of Medicine, Washington University School of Medicine , St. Louis, Missouri, USA.
Journal of virology
|September 7, 2023
概括
SARS-CoV-2 BQ.1.1 变种在特定的动物模型中显示出增加的毒性,特别是那些表达人类ACE2 (hACE2) 的动物. 这凸显了对动物模型进行重新评估的必要性,以测试新的COVID-19对抗措施,以应对不断演变的变种.
科学领域:
- 病毒学和免疫学 病毒学和免疫学
- 传染性疾病 传染性疾病
- 疾病的动物模型.
背景情况:
- 新出现的严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 变种挑战疫苗和治疗疗效.
- 对动物模型的重新评估对于评估针对新型SARS-CoV-2变种的对策至关重要.
研究的目的:
- 在各种动物模型中评估SARS-CoV-2 Omicron BQ.1.1变异的致病性.
- 为了比较BQ.1.1与BA.5.5变体在K18-hACE2转基因小鼠,常规小鼠和仓鼠中的毒性.
主要方法:
- 接种K18-hACE2转基因小鼠,C57BL/6J小鼠,129S2小鼠和叙利亚仓鼠的SARS-CoV-2 BQ.1.1和BA.5.5变种.
- 评估减肥,肺中的病毒复制,肺病理和临床疾病.
- 对子的直接接触和空气传播的评估.
主要成果:
- 与BA.5.5.5.2相比,BQ.1.1在K18-hACE2小鼠中引起了显著的体重减轻,更高的病毒复制和更大的肺病理.
- 在感染BQ.1.1与BA.5.5.5的C57BL/6J小鼠,129S2小鼠或仓鼠中没有观察到增加的呼吸道感染或疾病.
- 对于BQ.1.1和BA.5.5两种变种,子的传播率相似.
结论:
- 在HACE2-表达动物中,SARS-CoV-2 BQ.1.1变种表现出增加的毒性,可能是由于独特的尖峰突变.
- 传统的老鼠菌株和仓鼠对BQ.1.1和BA.5.5具有相似的敏感性,这表明病原性在模型特定的差异.
- 这些发现强调了选择适当的动物模型来评估新型SARS-CoV-2变种的影响和制定对策的重要性.
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