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白尾鹿的结构基础,Odocoileus virginianus,ACE2识别了所有高亲和度的SARS-CoV-2变种
Pu Han1, Yumin Meng1,2, Di Zhang1,3
1CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS) , Beijing, China.
Journal of virology
|September 7, 2023
概括
白尾鹿ACE2强烈与SARS-CoV-2变种结合,表明潜在的野生动物储库. 持续监测至关重要,以防止从鹿种群的病毒溢出事件回到人类.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 野生动物健康 野生动物健康
背景情况:
- SARS-CoV-2 已经显示出不断扩大的宿主范围,白尾鹿 (WTD) 已成为一个重要的野生动物储备区.
- WTD可能含有不再在人类群体中流通的令人担忧的变体 (VOC),构成重新引入的风险.
研究的目的:
- 综合评估WTD血管酶转化酶2 (ACE2) 对来自各种菌株的SARS-CoV-2尖端 (S) 受体结合域 (RBD) 的结合亲和力.
- 阐明WTD ACE2和SARS-CoV-2 RBD之间相互作用的分子机制,包括不同的变体.
- 评估从WTD种群到人类的SARS-CoV-2溢出事件的风险.
主要方法:
- 进行了结合性试验,以评估WTD ACE2与原型和变种菌株的SARS-CoV-2 S-RBD之间的相互作用.
- 使用结构生物学技术确定了WTD ACE2与原型,Omicron BA.1和BA.4/5 S剪切器的复杂结构.
- 进行了详细的结构比较,以确定参与WTD ACE2结合的关键氨基酸残留物.
主要成果:
- WTD ACE2被所有测试的SARS-CoV-2 RBDs广泛识别.
- 结构分析显示,RBD (486,498和501位) 中的特定残留物对于结合WTD ACE2至关重要.
- 该研究确定了Omicron BA.1和BA.4/5变体与WTD ACE2结合的结构基础.
结论:
- 这些发现凸显了SARS-CoV-2在WTD种群中传播和演变的潜力,作为人类再感染的储存库.
- 了解SARS-CoV-2变体和WTD ACE2之间的分子相互作用对于评估溢出风险至关重要.
- 持续监测野生动物群体中SARS-CoV-2感染的情况,特别是WTD,对于减轻公共卫生威胁至关重要.
关键词:
在ACE2中,ACE2是ACE2.它们是Odocoileus virginianus的在RBD中,RBD是RBD.S蛋白质是S蛋白质的组成部分.这就是SARS-CoV-2病毒.化-EM结构的结构.白尾鹿是一种白尾鹿.更多相关视频
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