耳鼻和鼻的年龄相关的组织学和生物化学变化
Sul Gi Kim1, Deanna C Menapace2, Mark M Mims3
1University of North Carolina School of Medicine, Chapel Hill, North Carolina, U.S.A.
The Laryngoscope
|September 7, 2023
概括
衰老会减少人类耳朵和鼻的糖氨基酸和细胞数量. 原蛋白随着年龄的增长而增加,可能会降低这些重要的面部软骨的结构完整性和灵活性.
科学领域:
- 生物化学 生物化学
- 历史学 历史学 历史学
- 衰老研究研究 衰老研究
背景情况:
- 人类耳部软骨 (AC) 和鼻子软骨 (四角软骨 (QC) 和背面隔膜关节 (DSA)) 对面部结构和功能至关重要.
- 这些软骨的生化和组织学性质的与年龄相关的变化尚未完全理解.
- 了解这些变化对于重建性手术和管理与年龄相关的美学变化至关重要.
研究的目的:
- 分析人耳和鼻的组织学和生物化学特征的与年龄相关的变化.
- 为了量化糖氨基 (GAGs),弹性素,原蛋白和细胞数量随着年龄的增加而发生的变化.
- 为了将这些生化变化与对软骨结构完整性的潜在影响相关联.
主要方法:
- 一项前性横截面研究,涉及73名活着的软骨捐赠者 (年龄20-77岁).
- 组织化学染色 (Safranin O,Verhoeff's,Masson's三色素) 用于评估GAG,弹性素和原.
- ImageJ2软件用于细胞计数和染色面积量化.
- 使用R研究室进行统计分析,将年龄与生化和细胞变化相关联.
主要成果:
- 在DSA亚站点观察到与年龄相关的GAG显著下降 (p < 0.001).
- 在DSA (p < 0.001) 和QC (p < 0.05) 亚站点中,与年龄相关的原蛋白含量显著增加.
- 在所有研究的软骨子部位上,软骨细胞数量与年龄一起下降.
结论:
- 衰老与耳鼻和鼻的GAG含量和冠状细胞细胞数量的减少有关.
- 这些软骨中随着年龄的增长而增加的原蛋白含量可能会导致结构完整性和灵活性降低.
- 这些发现为影响软骨功能和形式的与年龄相关的变化提供了组织学证据.
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