亚罗维亚脂质菌的代谢工程用于山丁生产
Guilin Zhang1,2, Jing Chen1,2, Yongzhen Wang1,2
1Qingdao Key Laboratory of Food Biotechnology, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
Journal of agricultural and food chemistry
|September 7, 2023
概括
这项研究为*Yarrowia lipolytica*进行了高效的山丁生产的工程. 代谢工程策略显著增加了山丁产量,达到775.3 mg/L.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物生产 微生物生产
背景情况:
- 泽亚桑丁是一种具有抗氧化和神经保护性质的胡卜素,是一种有价值的化合物.
- 有效和可扩展的山丁生产方法对于其应用至关重要.
- 酵母菌 (Yarrowia lipolytica) 是微生物生产有价值化合物的有希望的宿主.
研究的目的:
- 为了工程 *Yarrowia lipolytica* 高产量泽丁生产.
- 为了优化Y. lipolytica*中的胡卜素生物合成途径.
- 为了实现一个商业上可行的生产水平的泽亚桑丁.
主要方法:
- 在 *Yarrowia lipolytica* PO1h. 中的胡卜素路径的工程.
- 引入异质基因 (*mvaE*, *mvaS*, *crtZ*) 来增强前体供应和山丁合成.
- 使用野生和突变形式和模块化组装优化酶活性.
主要成果:
- 通过路径调节,β-胡卜素度从19.9升至422.0毫克/升.
- 通过引入美酸盐路径基因,进一步提高β-胡卜素产量至536.8 mg/L.
- 通过结合β-胡卜素氧化酶 (*crtZ*) 和氧化还原酶 (RFNR1) 来达到775.3毫克/升的最终泽亚桑产量.
结论:
- *Yarrowia lipolytica*的新陈代谢工程是高水平山丁生产的有效策略.
- 这种工程菌株显示出对工业规模的山丁制造有很大的潜力.
- 这项研究为酵母中的胡卜素生物合成提供了一个强大的平台.
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