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来自番茄黄色叶子卷曲病毒的新型C5蛋白是一种毒性因子和抑制基因沉默的抑制剂

Siwen Zhao¹, Pan Gong¹, Yanxiang Ren¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

|September 7, 2023

概括

番茄黄色叶子卷曲病毒 (TYLCV) C5蛋白是一种新发现的毒性因子. 这项研究表明,C5抑制了RNA沉默,并增强了植物病毒疾病的发展.

科学领域:

植物病毒学植物病毒学
分子生物学分子生物学
植物病理学植物病理学

背景情况:

番茄黄色叶子卷曲病毒 (TYLCV) 编码了六种正规蛋白质.
其他具有潜在毒性功能的小蛋白可能被TYLCV.编码.
在补充意义上的第五个开放阅读框架 (ORF),被指定为C5,是保存的,但它的功能是未知的.

研究的目的:

为了研究病毒感染期间TYLCV C5蛋白的表达和功能.
为了确定C5是否作为一种病原性决定因素和RNA沉默抑制剂.

主要方法:

通过促进体活性分析和定制抗体确认C5表达.
通过Nicotiana benthamiana的土豆病毒X (PVX) 载体表达C5的子宫外表达.
对C5对基因沉默 (PTGS和TGS) 和病毒复制的影响的分析.
在TYLCV.中对C5的突变和补充研究.

主要成果:

在感染细胞中证实了C5的表达.
宫外C5表达导致马赛克症状,增加病毒积累和反应性氧物种 (ROS) 爆发.
C5抑制了单链GFP诱导的PTGS和逆转的GFPTGS.
C5突变抑制了TYLCV的复制和疾病的发展.
C5过度表达补充了一个功能障碍的C5突变.

结论:

TYLCV C5 是一种致病性决定因素.
TYLCV C5 作为一种RNA沉默抑制剂.
这项研究扩大了对TYLCV蛋白质组功能能力的理解.

关键词:

C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 C5 过敏反应是一种过敏反应.致病性的决定因素.在转录后的基因沉默.番茄黄色叶子卷曲病毒病毒转录基因沉默是一种转录基因沉默.

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