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作为TIR催化信号分子的双层受体复合体的EDS1模块,以激活植物免疫

Jia Li¹, Xiaorong Tao²

¹The Key Laboratory of Plant Immunity, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

|September 7, 2023

概括

植物免疫受体使用TIR域来创建激活防御的信号分子. 这些分子有选择地触发通过EDS1,PAD4和SAG101蛋白质调解的独特免疫路径.

科学领域:

植物免疫力植物免疫力
分子生物学分子生物学
生物化学生物化学

背景情况:

植物细胞内核酸结合氨酸丰富的重复 (NLR) 受体与Toll/Interleukin-1受体 (TIR) 域检测病原体.
含有TIR的NLR (TNL) 信号涉及增强疾病易感性1 (EDS1) 和其合作伙伴 (植物素缺陷4[PAD4]或衰老相关基因101[SAG101]).
辅助NLRs,包括N需求基因1 (NRG1) 和激活抗病1 (ADR1),对于TNL信号传递至关重要.

研究的目的:

为了阐明由TIR-domains产生的特定信号分子.
为了识别这些信号分子的受体复合体.
了解这些分子如何激活不同的下游免疫路径.

主要方法:

生物化学测试用于检测和表征信号分子.
蛋白相互作用研究以确定受体复合体.
基因分析以确认信号分子和受体在植物免疫中的作用.

主要成果:

含有TIR的蛋白质催化了两个不同的信号分子的产生:ADPR-ATP/diADPR和prib-AMP/ADP.
EDS1-SAG101和EDS1-PAD4复合体分别作为ADPR-ATP/diADPR和prib-AMP/ADP的特定受体.
这些信号分子在全质上促进相互作用,从而激活了不同的EDS1-介导的免疫信号分支,包括NRG1和ADR1.

结论:

由TIR蛋白质产生的两个不同的小分子选择性地激活下游免疫信号通路.
这一发现促进了对TNL受体信号传递和植物免疫反应的理解.
这些发现揭示了通过特定的信号分子和受体复合体调节植物免疫力的复杂机制.

关键词:

在EDS1中使用EDS1.在NLR受体中,NLR受体是最重要的.在PAD4中使用PAD4.植物免疫力植物免疫力这就是SAG101的重点.信号分子的信号分子托尔/互乐金-1受体领域

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