果病原体 (Valsa mali) 的两个类似NIS1的蛋白质在植物识别和病原体毒性方面发挥着不同的作用
Jiajun Nie1, Wenjing Zhou1, Yonghui Lin1
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China.
Stress biology
|September 7, 2023
概括
来自Valsa mali的NIS1类蛋白VmNIS1和VmNIS2在植物相互作用中具有不同的作用. VmNIS1触发了植物免疫力,而VmNIS2则抑制了它,影响了病原体的毒性.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 菌效应生物学的生物学
背景情况:
- 植物病原体保护作用因子对于植物微生物相互作用至关重要.
- 类似NIS1的蛋白质是一种新发现的效应因子家族,其功能在很大程度上是未知的.
- 瓦尔萨马利会引起果瓦尔萨癌症,需要对其毒性因素进行研究.
研究的目的:
- 描述来自Valsa mali的两个类似NIS1的蛋白质VmNIS1和VmNIS2的功能.
- 研究它们在植物免疫反应和病原体毒性中的作用.
- 阐明它们与植物防御系统相互作用的基础机制.
主要方法:
- 在Nicotiana benthamiana中进行农业透,以评估VmNIS1和VmNIS2对细胞死亡和免疫的影响.
- 重组蛋白治疗,以评估VmNIS1对植物防御和抗病能力的影响.
- 在Valsa mali中进行基因淘汰,以确定VmNIS1和VmNIS2对于毒性和耐压力的必要性.
主要成果:
- VmNIS1诱导细胞死亡并增强植物对Phytophthora capsici的免疫力.
- VmNIS2抑制了植物免疫力,并促进了P. capsici感染.
- 只有VmNIS2淘汰赛影响了Valsa mali的毒性和氧化应激耐受性.
- VmNIS1的C端对植物识别至关重要;VmNIS2可能通过截断逃避检测.
结论:
- 在植物识别和病原体毒性方面,VmNIS1和VmNIS2的作用相反.
- 这些发现为NIS1-类蛋白质的功能多样性提供了新的见解.
- 了解这些效应因素可以为管理果Valsa癌症的策略提供信息.
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