对野外样本上子cadang-cadang viroid变异的分析,这些样本显示色斑点症状表现和严重程度的变化
Nur Diyana Roslan1, Shamala Sundram1, Lau Wei Hong2
1Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
Molecular biology reports
|September 7, 2023
概括
在棕油中发现了子cadang-cadang病毒的序列变异 (CCCVd). 然而,没有发现CCCVd变体,疾病严重程度或感染的手掌中的病毒积累之间的直接相关性.
科学领域:
- 植物病理学 植物病理学
- 分子病毒学分子病毒学
- 农业科学 农业科学
背景情况:
- 色斑点-子卡丹-卡丹病毒 (OS-CCCVd) 在油棕造成重大经济损失.
- OS-CCCVd的序列变化与症状变化有关,但仍未得到充分研究.
- 需要阐明CCCVd变体,疾病严重程度和病毒积累之间的关系.
研究的目的:
- 通过测序来表征受感染的油棕中的CCCVd变异.
- 为了将已识别的CCCVd变异与观察到的色斑点 (OS) 症状严重程度相关联.
- 调查受影响的手掌中CCCVd变异的积累水平.
主要方法:
- 从棕油叶样本中提取核酸.
- 使用聚烯胺凝电泳 (PAGE) 进行了分离.
- 检测和表征涉及逆转录聚合酶链反应 (RT-PCR),克隆,测序和实时定量PCR (qPCR).
主要成果:
- 鉴定出了CCCVd变异,其中一些与CCCVd_OP246完全相同,而另一些则呈现小的序列变异.
- 根据斑点大小和形态学,OS症状被分类.
- 疾病严重程度和CCCVd变异的积累之间没有直接相关性.
结论:
- 这项研究报告了CCCVd RNA的序列变化以及受OS影响的油棕树实地样本中的相关症状变化.
- 这些发现表明,疾病的严重程度与CCCVd变异的丰富程度没有直接关系.
- 进一步的研究可能会探索其他影响OS-CCCVd症状表现和疾病进展的因素.
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