瓦尔萨马利的VmPacC介导的pH调节赋予了通过比较蛋白质组学分析发现的宿主酸化
Liangsheng Xu1, Hailong Liu2, Shan Zhu2
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China. liangsheng.xu@nwafu.edu.cn.
Stress biology
|September 7, 2023
概括
瓦尔萨马利是一种致果癌的真菌病原体,降低了组织的pH值以防感染. 这项研究揭示了pH信号转录因子VmPacC调节能量代谢途径,这对于Valsa mali mali至关重要.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
背景情况:
- 果癌是由瓦尔萨马利引起的,导致了中国的重大经济损失.
- 瓦尔萨马利使宿主组织酸化到3.5的pH,以成功殖民.
- pH信号转录因子VmPacC对于V. mali的酸化能力和毒性至关重要.
研究的目的:
- 通过使用定量蛋白质组学来研究VmPacC介导的V. mali的pH调节.
- 在VmPacC删除和不同pH条件下识别差异表达蛋白 (DEP).
- 阐明VmPacC在宿主感染期间调节代谢途径中的作用.
主要方法:
- 双重质量标签 (TMT) 标签与基于LC-MS/MS的定量蛋白质组学相结合.
- 在VmPacC删除突变体和不同pH条件下 (pH 6.0和3.4) 分析DEP.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析,以及蛋白质-蛋白质相互作用 (PPI) 网络分析.
主要成果:
- 在不同的pH条件下确定了222个特定于VmPacC删除的DEP和921个DEP.
- DEP主要涉及碳代谢,抗生素生物合成,TCA循环,糖解/葡萄糖生成和其他代谢途径.
- 确定了119个共享的DEP,主要与能量代谢有关,其中关键蛋白质如热冲击蛋白60和阿斯巴酸氨基转移酶是网络的核心.
结论:
- VmPacC通过控制能量代谢途径,包括糖解/葡萄糖生成和TCA循环来调节V. mali的环境pH值.
- 提出了一个分子调节网络,用于V. mali的pH值下降.
- 这项研究提供了关于V. mali的pH调节机制的见解,有助于了解感染期间宿主酸化.
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