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在丝虫 (Bombyx mori) 中使用多omics识别与丝腺大小相关的基因

Lindan Sun¹, Binbin Sun¹, Liang Chen¹

¹School of Life Sciences, Jiangsu University, Zhenjiang, China.

Insect molecular biology

|September 7, 2023

概括

这项研究确定了调节丝虫丝腺大小和丝生产的关键基因. 四个已识别的基因与新陈代谢和生长有关,为改善丝产量提供了洞察力.

科学领域:

基因组学就是基因组学.
蛋白质组学是指蛋白质组学.
生物化学生物化学

背景情况:

在Bombyx mori中的丝腺大小直接影响丝产量.
丝腺大小的遗传调节的分子基础尚不清楚.

研究的目的:

识别与丝虫丝腺大小变异相关的基因和蛋白质.
阐明将丝腺体重与丝产量联系起来的分子机制.

主要方法:

在不同丝腺重量的丝虫菌株上进行比较的转录和蛋白质组学.
权重基因相关性网络分析以确定关键基因.
反转录定量实时聚合酶链反应 (RT-qPCR) 和酶相关免疫吸收试验 (ELISA) 进行验证.

主要成果:

在丝虫菌株之间确定了830和469个差异表达基因 (DEG).
检测到与代谢和生物合成过程相关的372和302个差异表达蛋白 (DEP).
五个基因与丝腺体重有显著关联;四个基因在较重的腺体表达更高,与新陈代谢有关.

结论:

参与糖原代谢,脂肪酸合成和分支链氨基酸代谢的四个特定基因对于丝腺生长和丝蛋白质合成至关重要.
这些发现为了解和潜在地增强丝虫丝生产提供了分子基础.

关键词:

多种主题的多种主题.蛋白质组学蛋白质组学丝腺的重量丝腺的重量丝虫是一种丝虫.翻译学翻译学翻译学翻译学

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