进化保存的圆形MEF2ARNAs调节肌体分化和骨肌肉发育
Xiaoxu Shen1,2, Xiyu Zhao1,2, Haorong He1,2
1Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
PLoS genetics
|September 7, 2023
概括
发现了两种来自MEF2A (circMEF2As) 的新型循环RNA,它们被证明可以促进动物的骨肌肉生长和肌体差异化. 这些circRNAs与MEF2A形成一个积极的反循环,影响肌肉发育.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 是骨肌肉发育的关键调节者.
- 肌细胞增强因子2A (MEF2A) 是肌细胞发生的关键转录因子.
- MEF2A衍生的circRNAs在肌肉发育中的作用尚不清楚.
研究的目的:
- 在肌肉干细胞中识别和表征MEF2A衍生的circRNAs (circMEF2As).
- 阐明circMEF2As在肌肉形成中的功能和调节机制.
- 研究线性MEF2A,circMEF2As和肌源性调节通路之间的相互作用.
主要方法:
- 在和小鼠肌肉干细胞中识别circRNAs.
- 在C2C12细胞和体内骨肌模型中的功能测试.
- 对miRNA-目标相互作用和基因表达的分析.
- 研究转录调节和反循环的研究.
主要成果:
- 确定了两种保存的circMEF2A (circMEF2A1和circMEF2A2). 这两种环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形环形.
- circMEF2A1通过miR-30a-3p/PPP3CA/NFATC1通路促进肌体发生.
- circMEF2A2通过miR-148a-5p/SLIT3/ROBO2/β-catenin通路促进了肌细胞分化.
- circMEF2As在体内增强了骨肌肉的生长.
- 建立了一个积极的反循环,涉及线性MEF2A,circMEF2As和miRNA.
- circMEF2As对于C2C12细胞的肌性分化至关重要.
结论:
- circMEF2As是进化保存的动物肌体发生的调节者.
- circMEF2As通过特定的分子途径在促进骨肌肉发育方面发挥着独特的作用.
- circMEF2As是自我调节的MEF2A反循环的组成部分.
- 这些发现提供了有关动物农业和人类医学的骨肌肉生物学的见解.
更多相关视频
相关概念视频
01:13Formation of Muscle Fibers from Myoblasts
5.0K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.0K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
01:21Satellite Stem Cells and Muscular Dystrophy
2.0K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.0K
02:02Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
01:23Regulation of Expression at Multiple Steps
942
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
942