一种基于大豆莱西纳米颗粒的扩展剂有效地冷保存了霍尔斯坦公牛的精子
Seyyed Mojtaba Mousavi1, Armin Towhidi1, Mahdi Zhandi1
1Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Animal reproduction science
|September 7, 2023
概括
纳米lecithin (NL) 显著改善了公牛精子的冷保存. 3%纳米莱西 (NL3) 的最佳度增强了运动性,活力和速度,显示了与蛋黄延长剂相似的结果.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
背景情况:
- 植物性精液扩展剂在稳定性和调制方面比动物性选择具有优势.
- 大量的乙素颗粒可以对牛精液扩展剂的溶解性和有效性产生负面影响.
- 使用超声波技术减少大豆莱素颗粒大小,产生纳米莱素 (NL).
研究的目的:
- 研究莱西类型和度对冷解牛精子质量的影响.
- 测试该假设,减少莱西颗粒大小可以增强精子扩展器相互作用,提高运动性,活力和生育能力.
- 为了比较纳米莱西 (NL) 与常规莱西和已知扩展剂的疗效.
主要方法:
- 来自霍尔斯坦公牛的精液使用Tris基扩展剂冷保存,其度不同 (1-4%) 常规莱西丁或纳米莱西丁 (NL).
- 对照扩展剂包括蛋黄 (EY) 和一种商用基于莱西的扩展剂 (BioXcell®).
- 评估了精子质量参数 (运动性,速度,活力,线粒体活动) 和体外受精 (体外受精) 结果 (裂变,化,胚芽细胞率).
主要成果:
- 3%的纳米莱西扩展剂 (NL3) 显示出优越的总和渐进性精子运动性和速度,与蛋黄 (EY) 相比.
- 精子线粒体活性在NL3中最高,而精子活力在EY,NL3和常规莱西中最高,为4% (L4).
- 在试管婴儿中,NL3实现了与BioXcell®相似的分裂和化率,但比EY更低的胚胎细胞率.
结论:
- 将莱西丁颗粒大小减少到纳米级,显著提高了牛精子冷保存.
- 3%纳米莱西 (NL3) 的最佳度为精子质量和冷生存提供了好处.
- 纳米lecithin扩展剂显示承诺作为传统蛋黄和商业扩展剂的有效替代品在公牛精液保存.
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