受到YY1调节的lncRNA SOCS2-AS1通过miR-454-3p/CPEB1抑制HCC细胞干细胞和进展
1Department of General Surgery, Hospital of Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
Biochemical and biophysical research communications
|September 7, 2023
概括
长非编码RNA SOCS2-AS1抑制肝细胞癌 (HCC) 的发生和进展. 这一发现为改善HCC治疗结果提供了潜在的治疗目标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 癌症干细胞 (CSC) 驱动肝细胞癌 (HCC) 复发和治疗耐药性.
- 了解CSCs的调节机制对于开发有效的HCC疗法至关重要.
研究的目的:
- 研究长非编码RNA (lncRNA) SOCS2-AS1在调节HCC细胞干性的作用.
- 阐明SOCS2-AS1在HCC进展中的功能背后的分子机制.
主要方法:
- 流细胞计用于NANOG+ CSC分类,球体形成试验,生命力,迁移,入侵和亡试验.
- 双化酶记者测定证实了SOCS2-AS1,miR-454-3p和CPEB1mRNA的结合位点.
- 定量实时PCR,西斑,ChIP,EMSA和皮下异种移植模型,以验证体外和体内发现.
主要成果:
- 在NANOG+ HCC干细胞中,SOCS2-AS1表达低;高的SOCS2-AS1水平与更好的患者存活率相关.
- SOCS2-AS1 抑制了 HCC 干,迁移和入侵,通过调节 miR-454-3p/CPEB1 轴来增强西斯普拉丁的敏感性.
- YY1被确定为一种抑制SOCS2-AS1转录的转录因子,其敲除促进了HCC干性.
结论:
- 由YY1调节的lncRNA SOCS2-AS1抑制了HCC细胞干细胞和通过miR-454-3p/CPEB1通路的进展.
- SOCS2-AS1代表了肝细胞癌治疗的潜在治疗标.
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