BCp12/PLA组合:一种新型抗菌剂,向Mur家族,DNA旋转酶和DHFR
Yufang Li1, Xiaozhao Tang2, Zushun Yang2
1College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
International journal of food microbiology
|September 7, 2023
概括
自然的抗微生物BCp12和酸 (PLA) 通过破坏细胞膜和DNA来协同杀死金黄色细菌. 这种组合显示出作为一种食品防腐剂来控制病原体的承诺.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 自然抗微生物BCp12/酸 (BCp12/PLA) 组合表现出增强的抗菌活性.
- 需要阐明BCp12/PLA对黄金葡萄球菌 (S. aureus) 的精确协同抗菌机制.
研究的目的:
- 为了研究BCp12和PLA对S. aureus的协同抗菌机制.
- 评估BCp12/PLA作为食品防腐剂的潜力.
主要方法:
- 分数抑制度 (FIC) 的确定.
- 流细胞计和扫描电子显微镜 (SEM) 用于细胞膜完整性分析.
- 用于基因组DNA相互作用研究的光光谱学.
- 酶抑制试验向丁糖和DNA合成酶.
- 在巴氏杀菌牛奶中应用,以评估抗微生物药物的有效性.
主要成果:
- BCp12/PLA证明了对S. aureus的协同抗菌作用,其FIC为0.05.05.
- 联合治疗协同破坏了细菌细胞膜,导致结构损伤和细胞质泄漏.
- BCp12/PLA与基因组DNA协同相互作用,并向皮糖甘和DNA合成中的关键酶,包括mur酶,二叶酸减少酶和DNA旋转酶.
- 这种组合有效地抑制了巴氏消毒牛奶中的微生物生长.
结论:
- BCp12和PLA协同作用,破坏黄金色菌细胞膜并影响基因组DNA.
- 这种组合的目标是参与细菌细胞壁和DNA合成的必需酶.
- BCp12/PLA代表了一种有前途的新型防腐剂战略,用于食品系统对抗食源性病原体.
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