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MsCYP71是草抗旱的积极调节剂

Jia Liu¹, Kun Shi¹, Shaopeng Wang¹

¹College of Grass Science and Technology, China Agricultural University, Beijing, 100193, China.

Plant physiology and biochemistry : PPB

|September 7, 2023

概括

细胞染色体P450基因MsCYP71增强了的抗干旱能力. 它的表达水平可以预测.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
遗传学是一个遗传学.

背景情况:

细胞染色体P450 (CYP450) 蛋白质对于植物的生长,发育和应激反应至关重要.
了解涉及压力耐受性的特定CYP450基因对于作物改善至关重要.

研究的目的:

为了研究黄细胞染色体P450基因MsCYP71在抗旱能力中的作用.
探索MsCYP71作为木干旱耐受性的生物标志物的潜力.

主要方法:

在中克隆MsCYP71基因.
病毒诱导的基因沉默 (VIGS) 以减少MsCYP71的表达.
烟草中MsCYP71的异质过度表达.
在干旱压力下分析生理参数 (相对含水量,净光合作用率,叶绿素光度最大值 - Fm).
在45个子加入中对MsCYP71的表达分析.

主要成果:

MsCYP71的表达是由干旱压力诱导的.
沉默MsCYP71降低了的抗干旱能力,由减少的水含量,光合作用和Fm.
过度表达MsCYP71增强了转基因烟草的抗干旱性和Fm.
发现MsCYP71表达和木加入的抗干旱能力之间存在正相关性.
的加入是集群的,I组表现出更高的抗干旱能力,Fm和MsCYP71表达.

结论:

MsCYP71 作为木抗旱的积极调节剂.
MsCYP71表达可以作为一个可靠的指标来评估的抗干旱能力.
在I组中加入的子代表了培育耐旱品种的宝贵遗传资源.

关键词:

阿尔法尔法 (Alfalfa) 是一种植物.干旱干旱干旱干旱在Fm Fm上播放.MsCYP7171 在线观看 VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS

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