过氧增加脏损伤在脏缺血和重新输血在小鼠
Melissa J Kimlinger1, Tom J No2, Eric H Mace3
1From the Vanderbilt University School of Medicine.
Anesthesia and analgesia
|September 7, 2023
概括
手术期间的氧气水平显著影响损伤. 在小鼠中,高 (过氧) 和低 (缺氧) 氧气水平都会在缺血和再注射 (IR) 损伤后恶化功能.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 关键护理医学 关键护理医学
- 手术生理学 手术生理学
背景情况:
- 缺血和再注射 (IR) 损伤是术后急性损伤的重要原因.
- 氧是IR事件期间功能的一个关键调节器.
- 了解氧气的作用对于在手术期间减轻脏损伤至关重要.
研究的目的:
- 在小鼠模型中调查脏IR手术期间不同氧气度对术后功能和损伤的影响.
- 探索氧气对内射损伤影响的潜在机制.
主要方法:
- 小鼠接受了脏IR或假手术与机械通风在不同的吸入氧气 (FiO2) 分数:0.10 (低氧),0.21 (正常氧),0.60 (中度高氧),或1.00 (严重高氧).
- 预先用超氧化物清除剂tempol对小鼠进行治疗,以评估其保护作用.
- 通过血液尿素 (BUN) 评估功能,通过组织学评分,NGAL染色和基因表达 (Ho-1,Pgc1-α,Gpx-4) 评估损伤.
主要成果:
- 在性IR期间的高氧和低氧都显著损害了功能,并且与正常性相比损伤增加了.
- 在IR后的中度过氧,严重过氧和缺氧组中,血尿素度显著升高.
- 高氧化物诱导的损伤被tempol部分减弱,这表明超氧化基的作用.
结论:
- 在内射线手术期间,在高氧和低氧水平上给予氧气会加剧损伤并损害功能.
- 这些发现凸显了当前在接受手术的患者中使用氧气的潜在风险,特别是那些有脏IR风险的患者.
- 这项研究表明,精确的氧气管理对于预防外科手术期间急性损伤至关重要.
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