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益生菌对鱼类神经传递和应激弹性作用的潜在机制通过转录基因分析
Yoonhang Lee1, Thanh Luan Nguyen2, HyeongJin Roh1
1Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyong National University, Busan, South Korea.
Fish & shellfish immunology
|September 7, 2023
概括
益生菌 Lactococcus lactis WFLU12 增强鱼类的神经传递和生长,同时减少压力. 这项研究揭示了橄海中微生物群-肠-大脑轴的机制,改善了水产养殖潜力.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 鱼类免疫学 鱼类免疫学
- 神经科学是一个神经科学.
背景情况:
- 微生物群-肠-大脑轴影响中枢神经系统和压力调节.
- 在鱼类的这一轴上存在有限的研究.
- 益生菌显示出通过这个轴调节鱼类健康的潜力.
研究的目的:
- 为了研究益生菌对橄树叶中神经传递和压力的作用机制.
- 了解Lactococcus lactis WFLU12在调节鱼类微生物群-肠-大脑轴上的作用.
- 提供对水产养殖中的益生菌益处的转录基因洞察力.
主要方法:
- 两项试验 (实验室规模和现场规模) 使用带有和没有 Lactococcus lactis WFLU12 的橄鱼 (Paralichthys olivaceus).
- 肝脏样本的RNA-Seq分析以分析基因表达.
- 监测鱼类的生长和测量血清学参数 (AST,LDH,皮质醇).
主要成果:
- 在实验室试验中,益生菌的使用上调了与神经传递相关的基因 (htr3a,mao,ddc,ntsr1,gfra2).
- 在实地试验中,在益生菌养的鱼类中显著促进生长和降低压力标志物 (AST,LDH,皮质醇).
- 在对照鱼中,压力反应基因 (hsp70,hsp90B1,hspE1,prdx1,gss) 和转录调节器 (fos,dusp1,dusp2) 的升级调节,表明益生菌可以减轻压力.
结论:
- 益生菌调节神经传递,并通过微生物群-肠-大脑轴减轻鱼类的压力.
- 乳球菌 WFLU12对鱼类生长和压力管理具有显著的益处.
- 这项研究提供了对水产养殖中的益生菌疗效的机制性理解.
关键词:
亚斯巴酸氨基转移酶 (AST) 是一种皮质醇是一种皮质醇.鱼类 鱼类 鱼类 鱼类微生物群-肠道-大脑轴神经传递的神经传递益生菌 益生菌 益生菌在RNA-Seqq.这是一种血清胺.减轻压力减轻压力减轻压力更多相关视频
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