子宫液 细胞外囊泡 蛋白质在雌性周期期间发生变化
Johanna Piibor1, Andres Waldmann2, Keerthie Dissanayake3
1Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia.
Molecular & cellular proteomics : MCP
|September 7, 2023
概括
牛子宫液细胞外囊泡 (EVs) 在雌性周期期间显示出动态的蛋白质组变化. 补充黄素阶段EV显著改善了体外胚胎的发育,突出了它们在生殖成功中的作用.
科学领域:
- 生殖生物学 生殖生物学
- 蛋白质组学是指蛋白质组学.
- 细胞外囊泡 细胞外囊泡
背景情况:
- 子宫环境利用复杂的信号通路来进行生理调节.
- 子宫液 (UF) 中的细胞外囊泡 (EV) 在子宫细胞通信中起着至关重要的作用.
- 牛雌性周期期间UF-EV蛋白质组的动态变化及其对胚胎发育的影响需要进一步研究.
研究的目的:
- 为了研究牛UF-EV蛋白质组的依赖雌性循环的动态.
- 评估不同周期阶段的UF-EV对体外胚胎发育的影响.
主要方法:
- 基于射击枪定量蛋白质组学的质谱法用于比较FU-EV蛋白质组在雌性周期的0,7和16天.
- 来自不同雌性周期阶段的UF-EV被补充到牛胚胎培养中.
主要成果:
- 确定了159种不同丰富的UF-EV蛋白质,表明一个循环依赖的蛋白质组.
- 已确定的关键途径对子宫功能,胚胎发育和营养 (例如抗氧化活性,细胞粘附) 至关重要.
- 用叶相UF-EV补充剂显著增加了体外胚胎细胞的发育率,从25.0 ± 5.9%增加到41.0 ± 4.0% (p ≤ 0.05).
结论:
- 牛UF-EV在整个雌性周期的子宫传播中发挥着重要作用.
- 在雌性周期期间,UF-EV蛋白质组合会发生变化,影响胚胎发育.
- UF-EV具有改善牛体内胚胎生产的潜力.
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