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由过氧酸盐触发碳酸 anhydrase 的氧化修饰在小鼠和类风湿病患者中引发免疫反应

Ayse Senturk¹, Ahmet Alver², Murat Karkucak³

¹Macka Vocational School, Karadeniz Technical University, Trabzon 61750, Türkiye.

The American journal of the medical sciences

|September 7, 2023

概括

氧化应激会改变碳酸酶 (CA) 酶,改变免疫反应. 过氧酸盐修饰显著增加了类风湿性关节炎和Sjögren综合征患者的CA自身抗体.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
类风湿病学类风湿病学

背景情况:

碳酸无水酶 (CA) 是重要的金属酶,参与许多生理过程.
对CA的自身抗体与某些类风湿性疾病有关.
氧化应激越来越被认为是抗CA自身抗体形成的触发因素.

研究的目的:

研究氧化/化应激终产品如何改变小鼠的CA抗原性.
探索修饰CA自身抗体与类风湿性关节炎 (RA) 和Sjögren综合征 (SjS) 患者的氧化剂-抗氧化剂状态之间的联系.

主要方法:

已分离的人类CA I和CA II异酶被-4-hydroxynonenal (4-HNE),malondialdehyde (MDA) 和过氧 (PN) 修改.
给小鼠进行了修改CA的免疫接种,以评估抗原性变化.
在RA和SjS患者中测量了自身抗体标位和氧化剂-抗氧化剂标记物 (MDA,4-HNE,3-甲,SOD,GSH-Px).

主要成果:

用HNE和MDA修改CA降低了小鼠的抗原性.
在小鼠中,PN修改增加了CA抗原性.
与对照组相比,在RA和SjS患者中观察到PN修饰的CA自身抗体水平显著升高.

结论:

氧酸盐的修饰可以引起对CA异酶的免疫反应.
在RA和SjS患者中,PN修饰的CA自身抗体的高标位很普遍,这表明在这些疾病中发挥了作用.

关键词:

自动抗体是一种自身抗体.碳酸无水酶是一种碳酸无水酶.马隆迪阿尔化物氧化应激是一种氧化应激.过氧化酸盐的过氧化

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