通过针对性的长读序列测定揭示了替代拼接和替代多基解的协调
Zhiping Zhang1,2, Bongmin Bae2, Winston H Cuddleston2
1Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, Farmington, CT, USA.
Nature communications
|September 7, 2023
概括
研究人员探索了神经系统发育过程中替代拼接 (AS) 和替代多化 (APA) 是如何协调的. 他们发现,3'未翻译区域 (3'UTR) 长度显著影响磁带外子拼接,揭示了这些过程之间的关键联系.
科学领域:
- 分子生物学分子生物学
- 神经科学是一个神经科学.
- 基因组学就是基因组学.
背景情况:
- 替代拼接 (AS) 和替代多化 (APA) 是神经系统发育中的关键调节机制.
- 这些过程往往被独立研究,使得它们的协调不太清楚.
- 研究AS和APA之间的相互作用对于全面了解基因调节至关重要.
研究的目的:
- 为了研究磁带外子 (CE) 拼接和Drosophila中的APA之间的协调.
- 开发和应用一种用于同时分析CE拼接和APA的新方法.
- 识别CE拼接受3'未翻译区域 (3'UTR) 长度影响的基因.
主要方法:
- 开发了Pull-a-Long-Seq (PL-Seq),这是一个有针对性的长读序列方法.
- PL-Seq 结合了cDNA pulldown 和纳米孔测序,以实现成本效益的分析.
- 使用分析管道量化CE含量与替代3'端结合.
主要成果:
- 鉴定了基因与差异CE拼接基于连接到短与长3'UTRs.
- 证明删除长3'UTRs会改变上游CE拼接在短3'UTR异型.
- 表明ELAV蛋白损失对CE拼接有不同的影响,这取决于3'UTR连接性.
结论:
- 该研究强调了考虑AS事件与替代3'UTR之间的连接的重要性.
- CE拼接和APA之间的协调是基因表达的重要监管层.
- 这些发现为控制神经系统发育的复杂机制提供了新的见解.
相关概念视频
Alternative RNA Splicing
21.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.3K
RNA Splicing
56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Pre-mRNA Processing: RNA Splicing
5.3K
5.3K
Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K


