与其紫色-pericarp玉米和白色超甜玉米父母相比,与新型紫色-pericarp超甜玉米相关的序列变化
Apurba Anirban1, Ardashir Kharabian Masouleh2, Robert J Henry2,3
1Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, Australia. apurba.anirban@bot.jnu.ac.bd.
Molecular genetics and genomics : MGG
|September 7, 2023
概括
与标准玉米相比,开发出了一种新的紫色超级甜玉米系列"Tim1",与标准玉米相比,它具有明显的安托素生物合成和甜味基因. 基因分析揭示了特定的序列差异,影响了周围的颜色和内核的甜度.
科学领域:
- 植物遗传学 植物遗传学
- 玉米育种 玉米育种
- 生物化学 生物化学
背景情况:
- 从现有的玉米品种中开发出了一种新的紫色-pericarp超级甜玉米系列"Tim1".
- 了解其紫色和甜味的遗传基础对于育种应用至关重要.
研究的目的:
- 为了研究"Tim1"与其母系之间的安托西亚宁生物合成和甜味基因的序列差异.
- 为了确定控制"Tim1"紫色心膜和超甜的遗传因素.
主要方法:
- 编码DNA序列 (CDS) 和关键基因 (A1, Pr1, Pl1, B1, R1, sh2) 的促进子区域的比较测序.
- 使用B73玉米基因组 (NAM5.0) 作为序列分析的参考.
主要成果:
- "蒂姆1"和"哥斯达黎加"具有相似的A1,Pl1和R1基因序列,有助于紫色心周.
- B1基因显示了"Tim1"和"Tims-white"之间的相似性,这表明B1基因在周围肌肤颜色中的作用有限.
- "Tim1"中的Pr1基因促进体具有 (TA) 重复,表明功能缺陷,与"哥斯达黎加"相比,红色的周围肌颜色更红.
- 'Tim1'和'Tims-white'共享类似的sh2基因序列,与它们的超甜的表型一致.
结论:
- 开发的紫色甜玉米系"Tim1"具有独特的基因变异,在安托西亚尼生物合成和甜玉米基因.
- 这些遗传差异使"Tim1"从参考非甜玉米品种中区分开来,并提供了对其理想特征的见解.
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