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相关概念视频

Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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相关实验视频

Updated: Jul 17, 2025

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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抗体批量克隆 抗体批量克隆

Rico Ballmann1, Kai-Thomas Schneider1, Kristian Daniel Ralph Roth1

  • 1Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics, Braunschweig, Germany.

Methods in molecular biology (Clifton, N.J.)
|September 7, 2023
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种高通量克隆协议,用于快速选双价格格的抗体候选者. 这种新方法简化了双价抗体分子的生产和分析,以提高特性.

关键词:
批量克隆 批量克隆是一种在并行克隆的过程中,单链断片变量 (scFv) 是一个单链断片变量.高通量的高通量菌体显示器可以显示菌体.scFv-Fcc 的意思

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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

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科学领域:

  • 生物技术是生物技术.
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • 菌体显示对于抗体发现至关重要,最初的查使用单价单链可变片段 (scFv).
  • 鉴定抗体克隆的特征通常需要像IgG或scFv-Fc融合这样的双价格格式来进行准确的评估.
  • 目前生产双价抗体的方法包括对V区域基因进行繁的克隆程序,将其转化为表达载体.

研究的目的:

  • 开发一种高通量程序,以快速选双价格格的抗体候选者.
  • 为选择的抗体碎片建立并行克隆协议,将其转化为哺乳动物表达载体.
  • 为了实现高效的生产和分析双价抗体分子在一个96井板格式.

主要方法:

  • 一个新的高通量协议,用于将抗体碎片并行克隆到哺乳动物表达载体中.
  • 采用96井板格式,用于简化克隆和随后的抗体生产.
  • 在96孔板中生产和净化双价抗体分子 (例如,IgG,scFv-Fc).

主要成果:

  • 在双价格格式中成功建立了用于抗体查的高通量程序.
  • 证明了选择的抗体片段的并行克隆,将其转化为哺乳动物表达载体.
  • 能够快速生产和净化用于下游测定的双价抗体分子.

结论:

  • 开发的协议显著加速了抗体候选人的查和表征.
  • 这种高通量方法简化了双价抗体的生产,减少了劳动和时间.
  • 96井板格式为药物发现和研究提供了双价格格的抗体克隆的高效分析.