Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.

Frontiers in bioengineering and biotechnology·2026

Same author

Novel bispecific T-cell engagers overcoming acquired EGFR resistance.

mAbs·2026

Same author

Generation of Monoclonal Autoantibodies From Myelin Oligodendrocyte Glycoprotein-Specific Human B Cells Using an Optofluidic-Based Platform.

Neurology(R) neuroimmunology & neuroinflammation·2026

Same author

Do Not Let Them Fall-ot: Emerging Role of Transcatheter Therapies in Adult Congenital Heart Diseases.

JACC. Case reports·2026

Same author

Impact of Proportionality of Secondary Tricuspid Regurgitation on Outcomes After Tricuspid Transcatheter Edge-to-Edge Repair.

Circulation. Cardiovascular interventions·2026

Same author

Incidence, Predictors and Outcomes of Bleeding Following Transcatheter Tricuspid Valve Repair: The TriValve Registry.

JACC. Cardiovascular interventions·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

查看所有相关文章

Search research articles

相关实验视频

Updated: Jul 17, 2025

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

通过菌体显示绘制地图来绘制位.

Stephan Steinke¹, Kristian Daniel Ralph Roth¹, Ruben Englick¹

¹Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany.

Methods in molecular biology (Clifton, N.J.)

|September 7, 2023

概括

此摘要是机器生成的。

了解抗体-抗原相互作用对于开发诊断和治疗方法至关重要. 这项研究详细介绍了用于表位图绘制的菌体显示方法,确定单克隆抗体 (mAbs) 抗原上的特定结合区域.

关键词:

生物标志物生物标志物上层层是什么意思上层是什么意思在地图上绘制出地图的地图.单克隆抗体是一种单克隆抗体.这就是Panning的意义.帕拉托佩 (Paratope) 是一个可见的形状.体显示器是体显示器.

更多相关视频

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

相关实验视频

Last Updated: Jul 17, 2025

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

科学领域:

生物化学生物化学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

单克隆抗体 (mAbs) 是重要的生物工具,具有多种应用.
识别表皮质-抗原相互作用对于推进诊断,治疗和疫苗至关重要.
皮层映射技术对于了解免疫反应和抗体功能至关重要.

研究的目的:

为构建单个目标的寡类菌素库提供一个协议.
为表位图绘制概述一个菌体显示泛图程序.
为了促进抗体-抗原结合部位的表征.

主要方法:

菌体显示技术用于在菌体表面上呈现寡聚库.
构建一个单个目标的寡类菌素库.
抽取程序来选择与特定单克隆抗体结合的菌体.

主要成果:

该研究提供了一个详细的协议,用于生成和利用菌体显示库用于表位图绘制.
描述的方法可以识别由单克隆抗体识别的特定表位.
这种方法允许对抗原结合部位进行表征.

结论:

体显示器是用于表位图绘制的多功能和有效技术.
提供的协议有助于对抗体-抗原相互作用进行详细的分析.
这种方法支持改进生物产品的开发和对免疫反应的理解.