Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Benchmarking antibody discovery fidelity and reproducibility with an assay-locked residue fidelity index.

BioTechniques·2026
Same author

Sprinkling in extra validation for high-value PTMs and therapeutic Abs with MILKSHAKE Western blots and Sundae ELISAs.

New biotechnology·2025
Same author

Site-directed antibodies targeting driver mutations of the KRAS protein.

New biotechnology·2025
Same author

Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein.

New biotechnology·2023
Same author

Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of <i>Treponema pallidum</i> BamA (TP0326).

Frontiers in immunology·2023
Same author

Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols.

Methods in molecular biology (Clifton, N.J.)·2023

相关实验视频

Updated: Jul 17, 2025

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

21.0K

埃皮沃尔夫 (Epivolve):一种针对位点定向抗体的协议.

Xiaofeng Li1, Kezzia S Jones2, Felicity E Acca2

  • 1Abbratech, Inc., Branford, CT, USA. xli@abbratech.com.

Methods in molecular biology (Clifton, N.J.)
|September 7, 2023
PubMed
概括

研究人员现在可以使用Epivolve策略生成特定部位的抗体. 这种新的免疫向方法利用修改后的氨基酸来产生高亲和度抗体,这些抗体精确地结合到目标部位.

关键词:
抗体是对抗体的一种.生物制造是生物制造的一种.发生性发育 发生性发育体显示器是体显示器.网站导向的指导.特定地点的特定地点.

更多相关视频

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.6K
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

11.6K

相关实验视频

Last Updated: Jul 17, 2025

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

21.0K
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.6K
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

11.6K

科学领域:

  • 生物技术是生物技术.
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • 产生针对特定蛋白位点的抗体仍然具有挑战性.
  • 现有的方法难以精确地准氨基酸.

研究的目的:

  • 介绍Epivolve,这是一种创建特定部位抗体的新技术.
  • 详细说明使用Epivolve开发特定部位抗体的协议.

主要方法:

  • 埃皮沃尔夫使用一种改性氨基酸作为伪形成的免疫标.
  • 抗体 (Ab) 亲和度成熟技术用于高亲和度结合.
  • 菌体显示器用于进化针对特定氨基酸结合的抗体寄生虫.

主要成果:

  • 埃皮沃尔夫能够产生特定部位的抗体.
  • 这项技术有助于与特定的氨基酸结合,包括非同义的氨基酸.
  • 高亲和抗体是通过亲和成熟形成的.

结论:

  • 埃皮沃尔夫 (Epivolve) 提供了一种独特的策略,用于产生特定部位的抗体.
  • 这项技术克服了针对蛋白质特定氨基酸的局限性.
  • 描述了使用Epivolve开发局部特异性抗体的协议.