在Clostridioides difficile感染期间调节结肠免疫代谢反应可改善疾病严重程度和炎症
Nuria Tubau-Juni1, Josep Bassaganya-Riera1, Andrew J Leber1
1NIMML Institute, Blacksburg, VA, 24060, USA.
困难闭膜炎感染 (CDI) 是导致腹的主要原因之一. 用2-脱氧-D-葡萄糖 (2-DG) 向宿主糖解,通过保护肠道屏障,降低了CDI的严重程度和死亡率.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 代谢学 代谢学 代谢学
背景情况:
- 艰难菌感染 (CDI) 是与抗生素相关的腹的重要原因,复发率高.
- 目前对CDI的抗生素治疗有局限性,有15-35%的患者出现复发.
- 针对宿主反应的新型治疗策略是有效的CDI管理所需的.
研究的目的:
- 在小鼠模型中研究Clostridioides difficile感染 (CDI) 期间宿主结肠基因表达和代谢反应.
- 为了确定新的宿主代谢途径,对CDI具有治疗潜力.
- 评估向宿主糖解作为治疗CDI的疗效.
主要方法:
- 在CDI的10天小鼠模型中进行全面的全球结肠转录组学分析.
- 代谢分析,以确定急性感染和恢复期间的关键途径.
- 在体内给予2-脱氧-D-葡萄糖 (2-DG),以抑制CDI期间的糖解.
- 评估疾病严重程度,死亡率,结肠炎和肠道上皮细胞完整性.
主要成果:
- 在急性感染期间,CDI诱导了结肠基因表达的重大变化,在康复过程中正常化.
- 观察到双相代谢反应:在炎症峰值期间高调糖解,在恢复过程中主导线粒体代谢.
- 使用2-DG抑制糖解,显著降低了CDI的严重程度,降低了死亡率,并改善了结肠炎.
- 2-DG保护肠道上皮细胞免受C. difficile毒素引起的损伤,保持屏障完整性并减少炎症.
结论:
- 主体免疫代谢途径,特别是糖解,在CDI的发病过程中起着至关重要的作用.
- 用2-DG准宿主糖解是一种对CDI的有希望的治疗策略.
- 主体代谢途径的药理调节为CDI提供了新的治疗方式.
更多相关视频
05:41Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
Published on: April 6, 2022
08:37Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
Published on: April 21, 2015
相关概念视频
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Inflammatory Bowel Disease IV: Pharmacological Management
Pharmacologic...
Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Drugs for Treatment of Ulcerative Colitis in IBD
Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids
