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ABCC2刷边界表达预测皮肤状细胞癌的结果:对254例病例的多机构研究

Vincent Francis Castillo^1,2, Mehdi Masoomian^1,3, Kiril Trpkov⁴

¹Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.

|September 8, 2023

概括

在 papillary 细胞癌 (PRCC) 中,ABCC2 边缘表达与更高的基因表达相关,并独立预测患者的生存结果. 这一发现为PRCC提供了一个新的预后标记,超越了传统的分级系统.

科学领域:

在瘤学瘤学.
细胞癌研究细胞癌研究
分子病理学分子病理学

背景情况:

在2022年世卫组织分类中,不再推乳头性细胞癌 (PRCC) 组织学亚型.
目前,世卫组织/ISUP核分级是PRCC的唯一被接受的预后组织学参数.
作为脏药物载体的ABCC2已经显示出对PRCC的结果具有显著的预测价值.

研究的目的:

评估ABCC2免疫组织化学 (IHC) 染色模式在大型多机构PRCC队列中的预后意义.
评估ABCC2 IHC模式与ABCC2 mRNA表达之间的关联.

主要方法:

对ABCC2 IHC反应模式评估了254个PRCC:负,细胞质,刷边界<50%,刷边界≥50%.
利用RNA in situ杂交 (ISH) 来确定每个组的转录水平.
使用SPSS和GraphPad软件进行了生存分析.

主要成果:

与阴性/细胞质组相比,任何刷边界染色的ABCC2组显示出显著增加的转录水平 (P=0.034).
在单变量分析中,ABCC2刷边界<50% (P=0.024) 和≥50% (P<0.001) 两组都与较差的无病存活率 (DFS) 有关.
多变量分析证实了ABCC2 IHC刷边界反应性 (分别P=0.037和P=0.003) 和高阶段疾病 (P<0.001) 作为DFS的独立预测因素.

结论:

在PRCC中ABCC2 IHC边缘表达与显著更高的基因表达相关.
在ABCC2 IHC边缘表达独立预测PRCC的生存结果.
这种IHC标志物显示出预后意义,特别是在早期和较小的瘤中,在某些子组中表现优于WHO/ISUP等级.

关键词:

ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2ABCC2A 生物标志物生物标志物型细胞癌是细胞癌.预后预后预后

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