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暂时受体潜在通道在巨细胞激活过程中受到微泡的影响

Jia Liu¹, Long Qing², Yufei He¹

¹Department of Dermatology, Changhai Hospital, Second Military Medical University (The First Affiliated Hospital of Naval Medical University), Shanghai, China.

Journal of cellular and molecular medicine

|September 8, 2023

概括

微泡激活巨细胞,导致脱粒和流入. 通过向TRPV1和TRPV4通道,这一过程被显著抑制,这表明它们参与了微泡诱导的巨细胞激活.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物物理学的生物物理.

背景情况:

乳腺细胞在免疫反应中起着至关重要的作用.
微气泡被用于各种生物医学应用.
暂时受体潜力 (TRP) 道参与细胞信号传输.

研究的目的:

为了研究TRP通道在微泡诱导的巨细胞激活中的作用.
探索巨细胞脱粒和流入由微泡触发的机制.

主要方法:

骨髓衍生性巨细胞 (BMMCs) 用微泡刺激.
测量了脱原标记物 (β-hexosaminidase, histamine, CD63) 的使用情况.
用光显微镜和流动细胞计量来评估细胞内的流入.
使用TRP通道抑制剂 (TRPV1,TRPV4) 来阻止特定的通路.

主要成果:

微泡刺激显著增加了BMMC脱粒和流入.
抑制TRPV1和TRPV4通道的抑制剂显著减少了微泡诱导的脱粒化.
流量水平与脱粒能力相关.

结论:

暴露在微泡中会激活巨细胞,导致脱粒.
TRPV1和TRPV4通道在调解微泡诱导的巨细胞激活方面发挥着至关重要的作用.
在这个过程中,TRP道依赖的流是关键机制.

关键词:

和是最重要的.压力下降疾病是压力下降疾病.脱粒化和脱粒化的方法巨细胞巨细胞是什么微型气泡是一种微型气泡.过渡性受体潜在的潜在受体

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