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小肠和大肠上皮质之间的IL-23/IL-22-依赖基因表达对微生物群的差异依赖

Ayaka Nakatani^1,2, Ryu Okumura^1,3,4, Airi Ishibashi¹

¹Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

Genes to cells : devoted to molecular & cellular mechanisms

|September 8, 2023

概括

介质素-23/介质素-22 (IL-23/IL-22) 轴在小肠和大肠中不同地影响肠道屏障. 这个轴依赖于小肠中的微生物群,而不是大肠,明显影响脂质代谢和细胞增殖.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
分子生物学分子生物学

背景情况:

发自自身免疫细胞膜的介质素-23/介质素-22 (IL-23/IL-22) 轴,对于维持肠道上皮屏障至关重要.
这个轴促进抗微生物的产生和上皮细胞的增殖,这对肠道健康至关重要.
以前的研究表明,IL-23/IL-22轴在小肠和大肠中都有共同的功能,但特定位置的差异仍然不清楚.

研究的目的:

为了全面分析和比较肠道上皮细胞的基因表达,在大肠和结肠.
阐明IL-23/IL-22轴在小肠和大肠之间的上皮细胞上的功能相似之处和差异.
调查肠道微生物群在调解IL-23/IL-22轴功能的作用. 在不同的肠道部分.

主要方法:

用RNA测序分析了从无菌,Il23淘汰 (Il23^-/-) 和Il22淘汰 (Il22^-/-) 的小鼠的肠上皮细胞中的基因表达.
对从大肠 (小肠) 和结肠 (大肠) 的上皮细胞进行了比较分析.

主要成果:

IL-23/IL-22轴对肠道微生物群的依赖在肠道部位之间有显著差异;它在小肠中很大程度上依赖微生物群,但在大肠中不那么依赖.
在小肠中,IL-23和IL-22被发现对上皮细胞中的脂质代谢产生负面调节.
在大肠中,IL-23和IL-22因它们对上皮细胞增殖的积极调节而被突出.

结论:

IL-23/IL-22轴在维持肠道上皮细胞在大肠和结肠中的生理功能方面表现出不同的,特定于位点的作用.
这些发现揭示了前所未有的差异,即IL-23/IL-22轴在小肠与大肠之间如何运作,受到微生物群的影响,并影响不同的细胞过程.

关键词:

在IL-23/IL-22轴上.细胞的增殖细胞的增殖.我们的肠道微生物群.肠道上皮细胞肠道上皮细胞脂质新陈代谢脂质新陈代谢

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