基于GC-MS指纹,GC多元组件定量分析和化学模式识别分析的扬克混合物 (YM) 挥发性油的质量评估
Xiaolian Liang1,2, Zhengteng Yang3, Jiabao Ma3
1Guangxi University of Chinese Medicine, Department of Pharmaceutical Analysis, Nanning 530000, China.
Journal of chromatographic science
|September 8, 2023
概括
通过气色谱质谱法 (GC-MS) 来分析挥发性油,加强了扬扬克混合物 (YM) 的质量控制. 这种方法确定了在传统中医药中保持一致的YM质量的关键化学标志物.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 传统中国医药 传统中国医药
背景情况:
- 延安混合物 (YM) 是一种包含12种草药的传统中医药 (TCM),用于治疗喉炎症状,如咳和吐困难.
- 由于YM的复杂多组分性质,其广泛的临床使用需要强有力的质量控制措施.
研究的目的:
- 根据其挥发性油成分,为YM开发一个质量控制系统.
- 为YM挥发性油建立GC-MS指纹和含量确定方法.
主要方法:
- 使用蒸汽蒸从YM提取挥发性油.
- 使用GC-MS的化学成分分析和挥发性油的识别.
- 用GC-MS指纹建立,相似性评估和化学分析 (集群,PCA,PLS) 进行批量比较.
- 选择标记元件和开发GC含量确定方法.
主要成果:
- 在YM挥发性油中确定了43种化学成分.
- 为YM挥发性油建立了GC-MS指纹,并评估了其相似性.
- 利用化学测量方法,在不同的YM批次中比较挥发性油成分.
- 选择了三个关键成分作为潜在的活性标记物,并开发了量化它们的方法.
结论:
- 为YM挥发性油建立了一个快速,可靠和有效的质量控制方法.
- 开发的方法为YM研究和开发提供了有价值的参考.
- 这种方法为其他TCM处方的质量控制提供了一个新的策略.
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