一个分子框架,用于控制西红中花系统的局部发育
Hengzuo Xiang1,2,3,4,5, Sida Meng1,2,3,4,5, Yunzhu Ye1,2,3,4,5
1College of Horticulture, Shenyang Agricultural University, Shenyang, China.
Frontiers in plant science
|September 8, 2023
概括
形的西红果,与局部数相关,受LC基因的影响. 这项研究揭示了LC与SlSEP3和基因素脱乙酶相互作用,以调节SlWUS,影响番茄部位的发育.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 发育生物学是发展生物学.
背景情况:
- 有多个位置的形番茄果实是影响质量的常见问题.
- 众所周知,定量特征位点 (QTLs) lc 和 fas 影响番茄位点数.
- 在体发育过程中LC的精确分子机制尚不清楚.
研究的目的:
- 为了研究番茄果实发育的LC基因的分子机制.
- 确定与loc相互作用的蛋白质,并阐明它们在调节loccule数量中的作用.
主要方法:
- 产生的LC淘汰突变体和SlSEP3沉默装置.
- 执行了cDNA库的构建和酵母的一种混合选.
- 进行了酵母双杂交测试,以确定相互作用的蛋白质.
主要成果:
- 如果淘汰突变者表现出增加的花器官和水果部位,具有较高的SlWUS表达.
- 确定SlSEP3作为lc.的有约束力的合作伙伴.
- 通过对SlSEP3进行静音化,Lc突变的表型得到了复制.
- 发现了SlSEP3和组织素脱乙酶 (SlHDA1-8) 之间的相互作用.
结论:
- 通过SlSEP3与基因素脱乙酶相互作用,将其招募到通过LC位点抑制SlWUS转录.
- 这种机制调节核细胞松,影响SlWUS表达和番茄位数.
- 揭示了一种涉及lc,SlSEP3和素脱乙酶在番茄部发育中的新型遗传途径.
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