在光系I中能量转移和捕获,含有和不含有叶绿素
Ivo H M van Stokkum1, Marc G Müller2, Jörn Weißenborn1
1Department of Physics and Astronomy and LaserLaB, Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1081, Amsterdam 1081 HV, the Netherlands.
在远红光下 (FRL) 种植的紫菌光系统I (PSI) 显示出由于上坡能量转移而延迟的能量捕获. 这项研究揭示了与白光-PSI相比,FRL-PSI的不同光谱和运动性质.
科学领域:
- 光合作用研究研究光合作用.
- 生物物理学的生物物理.
- 蓝藻细菌的能量转移方式
背景情况:
- 光系统I (PSI) 对于蓝藻细菌的光合作用至关重要.
- 照明条件显著影响PSI结构和功能.
- 了解能量转移动态是优化光采集的关键.
研究的目的:
- 在PSI中建立能量转移和捕获的一般动态方案.
- 在白光 (WL) 和远红光 (FRL) 下解析PSI的光谱和运动性质.
- 为了阐明FRL培养的蓝菌中延迟捕获背后的机制.
主要方法:
- 同时对排放和短暂吸收数据集进行目标分析.
- 对五种蓝藻细菌菌株进行了光谱测量.
- 能量传输路径的动态建模.
主要成果:
- WL-PSI包括大量,两个红色和一个反应中心 (WL-RC).
- FRL-PSI包括两个额外的Chlf区,具有~29nm红移FRL-RC最低激发状态.
- 电荷分离速率从~900 ns-1 (WL-RC) 降至~300 ns-1 (FRL-RC).电荷分离速率从~900 ns-1 (WL-RC) 降至~300 ns-1 (FRL-RC).电荷分离速率从~900 ns-1 (WL-RC) 降至~300 ns-1 (FRL-RC).电荷分离速率从~900 ns-1 (WL-RC) 降至~300 ns-1 (FRL-RC).电荷分离速率从~900 ns-1 (WL-RC) 降至~300 ns-1 (FRL-RC)
结论:
- 在FRL-PSI (~130 ps) 捕获的延迟归因于从Chlf区块的上坡能量转移.
- 在FRL-PSI中的吉布斯自由能量略低于FRL-RC,导致了上坡转移.
- 该研究提供了在不同的光照条件下PSI能量动态的详细动态模型.
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