黑色素通过改善线粒体功能来增强老年生殖年龄妇女未成熟卵细胞的发育潜力
Dandan Yang1,2, Yaoqin Mu3, Jing Wang1,2
1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.81Meishan Road, Hefei, 230032, Anhui, China.
Heliyon
|September 8, 2023
概括
在体外成熟 (IVM) 期间补充黑素 (MT) 改善了老年妇女的卵细胞质量. 这种方法通过逆转与年龄相关的卵子质量下降来提高生育能力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学是细胞和分子生物学.
- 生物化学 生物化学
背景情况:
- 老龄化对人类卵细胞质量产生负面影响,影响更年长的生殖年龄妇女的生育能力.
- 试管成熟 (IVM) 是辅助生殖技术中的一个关键技术.
- 黑素 (MT) 是一种内源性抗氧化剂,对卵细胞质量有潜在的益处.
研究的目的:
- 研究在体外成熟 (IVM) 期间补充黑素 (MT) 对人类卵细胞质量与年龄相关的影响.
- 确定MT是否可以逆转与年龄相关的卵细胞质量下降.
主要方法:
- 一项涉及172名老年生殖年龄妇女 (≥35岁) 和83名年轻妇女 (<35岁) 进行体外受精 (IVF) 的研究.
- 未成熟的卵细胞接受了IVM,有或没有10μM MT.
- 评估了卵细胞受精,胚胎发育,线粒体功能 (免疫光,mtDNA复制号),以及螺旋/染色体组成 (免疫光,测序).
主要成果:
- 在老年妇女的IVM中等和成熟卵细胞中,MT补充改善了氧化还原水平.
- 在成熟卵细胞中增加了线粒体循环DNA比例和ATP含量.
- 增强了正常的线和染色体组成,从而改善了老化卵细胞的卵细胞成熟和胚胎细胞形成率.
结论:
- 在IVM期间补充黑素 (MT) 有效地逆转了与年龄相关的人类卵细胞质量下降.
- 这一发现为提高老年生育年龄妇女生育能力提供了潜在的策略.
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