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在PPRV感染后,山羊肺的m6ARNA甲基化的变化
Raja Ishaq Nabi Khan1,2, Manas Ranjan Praharaj3,4, Waseem Akram Malla1
1Division of Veterinary Biotechnology, ICAR - Indian Veterinary Research Institute, Izatnagar Bareilly, 243122, U.P., India.
Heliyon
|September 8, 2023
概括
小反动物瘟疫 (PPR) 改变了绵羊和山羊肺部的RNA甲基化 (m6A). 这些变化影响免疫信号通路,为疾病机制提供了洞察力.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 小反动物瘟疫 (PPR) 是一种严重的病毒性疾病,影响山羊和绵羊.
- 像N6-甲基氨酸 (m6A) 这样的RNA修饰在疾病过程中起作用.
- 肺组织是PPR病毒 (PPRV) 的首要目标.
研究的目的:
- 为了研究PPRV感染的肺组织中的m6ARNA修饰变化.
- 了解这些m6A变化在PPR发病过程中的功能意义.
主要方法:
- 在感染和正常的肺组织上进行了A-sequencing (m6A-seq).
- 分析了差异性m6A峰值和转录的表达水平.
- 对差异修饰和表达的基因进行了功能丰富分析.
主要成果:
- 在感染PPRV的肺部观察到m6A峰值的显著变化,其中低甲基化峰值多于高甲基化峰值.
- 低甲基化基因与介质素-4和介质素-13的信号传递和细胞外矩阵组织有关.
- 一个m6A-修改转录的子集也被差异表达,在免疫和细胞外矩阵通路中得到丰富.
- 关键的6个A监管机构 (HNRNPC,METTL14) 得到了监管的提升.
结论:
- 改变的m6景观是肺组织中PPRV感染的特征.
- 这些表观遗传变化与关键的免疫和细胞过程有关,包括Interleukin信号传递.
- 这些发现为了解PPR病变的产生提供了分子基础.
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