相关实验视频

Updated: Jul 17, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.4K

固态增强控制酶辅助DNA组装的控制

Oliver J Irving1, Lauren Matthews1, Steven Coulthard1

  • 1School of Chemistry, University of Birmingham Edgbaston, Birmingham, B15 2TT, UK.

Chembiochem : a European journal of chemical biology
|September 8, 2023
PubMed
概括

纯控核酶增强 (SCoNE) DNA组装克服了小型DNA片段的传统方法的局限性. 这种新的方法使得用于分析物检测的功能化DNA结构的创建成为可能,其性能优于传统的吉布森组件.

关键词:
DNA组装组件的组装吉布森组装组装公司生物标志物捕获 生物标志物捕获酶性修饰 酶性修饰

更多相关视频

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.3K
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

4.0K

相关概念视频

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.6K
Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
30.9K
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
31.4K