氧化铁纳米颗粒通过自途径通过与帕克利塔塞尔的协同捆绑诱导铁亡
Qi Nie1, Wenqing Chen1, Tianmei Zhang1
1Guangxi Clinical Medical Research Center for Neurological Diseases, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
Molecular medicine reports
|September 8, 2023
概括
装有帕克利塔塞尔 (IONP@PTX) 的氧化铁纳米颗粒通过铁灭作用协同诱导癌细胞死亡. 这种纳米疗法增强了铁离子度,活性氧物种和脂质过氧化,主要通过自途径.
科学领域:
- 生物医学工程 生物医学工程
- 纳米医学是一种纳米医学.
- 癌症治疗 癌症治疗
背景情况:
- 铁亡,一种受调节的细胞死亡形式,是癌症治疗的有希望的目标.
- 向纳米疗法为非侵入性癌症治疗提供了潜在的潜力.
- 在各种瘤中含有帕克利塔塞尔的氧化铁纳米颗粒 (IONP@PTX) 的机制需要阐明.
研究的目的:
- 为了准备IONP@PTX进行向癌症治疗.
- 探索IONP@PTX在抑制小细胞肺癌 (NCI-H446) 和质母细胞瘤 (M059K) 细胞中的机制.
- 调查自在IONP@PTX诱导的铁亡中的作用.
主要方法:
- 细胞活力测试 (CCK-8) 和组合指数评估.
- 测量细胞内活性氧物种 (ROS) 和脂质过氧化.
- 西方涂抹以评估与自和铁死相关的蛋白质表达 (贝克林1,LC3,p62,HDAC6,mTORC1).
主要成果:
- 与单一疗法或物理混合物相比,IONP@PTX在细胞活力上表现出协同作用.
- IONP@PTX显著增加了细胞内铁,ROS和脂质过氧化水平.
- IONP@PTX调节了与自相关的蛋白质 (贝克林1,LC3-II/I,p62) 和HDAC6的表达,拉巴素增强了这些效应.
结论:
- IONP@PTX有效地诱导癌细胞中的协同性铁亡.
- 观察到的铁亡是通过自途径的调节来调节的.
- IONP@PTX代表了一种有前途的纳米治疗策略,用于向癌症治疗.
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