塞拉蒂亚马尔塞森斯 (Serratia marcescens) 在惩戒设施的爆发:环境采样,实验室分析和基因组表征以评估来源和持久性
Donna Ferguson1, Rahil Ryder1, Rawni Lunsford1
1Public Health Laboratory, County of Monterey Health Department, Salinas, CA 93906, USA.
International journal of environmental research and public health
|September 8, 2023
概括
环境采样和基因组学在多年的惩戒设施爆发期间在清洁产品中确定了Serratia marcescens储存库. 疫情菌株持久且多样化,清洁剂充当关键的环境储存物.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- 塞拉蒂亚马尔塞森斯是一种机会性病原体,引起各种感染.
- 医疗保健和社区环境中的疫情带来了重大的公共卫生挑战.
研究的目的:
- 调查多年来在一个惩戒设施中爆发的Serratia marcescens.
- 确定疫情菌株的环境储存库和基因组特征.
主要方法:
- 环境采样和全基因组测序 (WGS).
- 对比基因组学和遗传学分析.
- 毒性因子的主要成分分析 (PCA).
主要成果:
- 从患者和与非法药物使用有关的物品中确定了一个主要的克隆相关的S. marcescens集群.
- 四级消毒剂 (CB64) 和多用途清洁剂 (BO) 充当了环境储水池.
- 疫情菌株表现出持久性和多样性,与全球菌株聚集在一起.
- 疫情菌株中抗菌素耐药性 (AMR) 基因之间没有相关性;NCBI菌株含有更多的AMR基因.
- 病毒性因子的变化观察到跨类群,而不是环境和临床菌株之间的变化.
结论:
- 环境水库,包括清洁剂,在维持S. marcescens的爆发中发挥着至关重要的作用.
- 基因组背景对于解释流行病学发现至关重要.
- 了解菌株的持久性和多样性有助于疫情控制策略.
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