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Updated: Aug 1, 2026

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
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参考基因U2允许直接比较血管光滑肌细胞在组织和培养中的相对基因表达水平,使用实时定量PCR

Christine Rager1,2, Tobias Klöpper1, Uwe Pfeil3

  • 1Signalling Transduction Group, Institute of Anatomy and Cell Biology, Faculty of Medicine, Justus-Liebig-University, 35392 Giessen, Germany.

Cells
|September 8, 2023
PubMed
概括

选择适当的参考基因 (RGs) 对于精确的基因表达分析在血管光滑肌细胞 (vSMCs) 中至关重要. 小核核核糖核蛋白U2 (snRNP U2) 被确定为可靠的,独立于性别的RG,用于比较培养的vSMC与原生组织中的vSMC.

关键词:
大动脉的动脉.qPCRR 是一个很好的方法.参考基因 (家庭管理基因)血管光滑肌肉细胞 血管光滑肌肉细胞

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