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缺氧诱导的HIF-1α促进了Listeria monocytogenes对鱼的入侵

Jing Wang¹, Zhan He¹, Mingzhu Cui¹

¹Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University , Hangzhou, Zhejiang, China.

Microbiology spectrum

|September 8, 2023

概括

低氧对鱼的HIF-1α进行上调,通过增加c-Met表达来促进Listeria monocytogenes的入侵. 这种机制解释了低毒鱼的细菌负担增加,为水产养殖病原体控制提供了洞察力.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
水生动物卫生水生动物卫生

背景情况:

低氧是一种低氧的状况,显著影响细胞功能和宿主-病原体相互作用.
李斯特菌 (Listeria monocytogenes) 是一种通过食物传播的病原体,可以感染各种宿主,包括鱼类.
鱼水产养殖在全球范围内至关重要,但容易受到细菌污染,构成公共卫生风险.

研究的目的:

在低氧条件下调查低氧诱导因子1-alpha (HIF-1α) 在Listeria monocytogenes入侵鱼中的作用.
阐明HIF-1α通过哪些分子机制影响鱼的细菌感染.

主要方法:

在低氧条件下,在鱼组织中量化HIF-1α表达.
感染测定与菌单细胞原体在低氧.
通过RNA干扰 (RNAi) 来调节HIF-1α的表达.
对c-Met受体表达的分析.
使用Listeria monocytogenes的一个∆InlB突变菌株来评估粘附和入侵.

主要成果:

在低氧鱼组织中,HIF-1α表达显著上调.
在所有低毒鱼的检查组织中,Listeria monocytogenes的细菌负担增加.
HIF-1α表达与c-Met表达正相关.
由InlB介导的入侵对鱼中Listeria monocytogenes感染至关重要.
低氧和HIF-1α对细菌计数的影响在∆InlB突变菌株中被废除.

结论:

HIF-1α通过上调c-Met表达来促进Listeria monocytogenes在低氧鱼中的入侵,从而促进细菌的内部化.
了解这种HIF-1α介导的途径,为控制水产养殖中的Listeria monocytogenes提供了洞察力.
这项研究有助于减轻受污染的鱼产品对公共卫生构成的威胁.

关键词:

李斯特里亚单细胞原生菌 (Listeria monocytogenes) 是一种低氧环境低氧环境这是入侵的入侵.蒂拉皮亚是因为它是蒂拉皮亚.

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