使用经济的双菌株培养和催化系统高效地生产α-甲酸
Kun Liu1, Yan Liu1, Xiangfei Li1
1Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, College of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, 241000, China.
Applied microbiology and biotechnology
|September 8, 2023
概括
一个新的双菌株系统有效地从L-glutamic acid (L-Glu) 中产生α-谷氨酸 (α-KG),使用工程E. coli. 这种方法过度表达酶以催化反应并消除有毒副产品,增强α-KG的产生.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么? 酶工程是什么
- 代谢工程是代谢工程.
背景情况:
- 传统的化学合成α-谷氨酸 (α-KG) 正被使用重组Escherichia coli过度表达L-谷氨酸氧化酶 (LGox) 的全细胞生物催化法所取代.
- 一个主要的挑战是催化酶 (Cat) 的共同表达,以消除有毒的副产品过氧化 (H2O2),这增加了细胞负担.
研究的目的:
- 从L-胺酸 (L-Glu) 制造α-KG的有效和经济的全细胞生物催化战略.
- 通过采用双菌株催化和培养方法来克服单菌株系统的局限性.
主要方法:
- 对Streptomyces platensis NTU3304 L-谷氨酸氧化酶 (SpLGox) 和Streptomyces lividans TK24催化酶 (SlCat) 的基因被插入到E. coli的低IPTG可诱导表达系统中.
- 建立并优化了一种双菌株催化系统,随后设计了一种双菌株培养策略,以简化扩大规模.
主要成果:
- 在特定条件下 (pH 7.0,35°C) 经过6个小时,优化的双菌株系统在特定条件下达到95.4g/L的α-KG标位.
- 与单菌株催化相比,生产率提高了97%,是迄今为止报告的最高生产率.
- 该策略有效地将L-Glu转化为α-KG,同时管理H2O2副产品.
结论:
- 开发的双菌株系统提供了一种高效且具有成本效益的方法,用于从L-Glu.产生α-KG.
- 这种方法通过简化扩展和提高产量来提高生物催化α-KG生产的工业适用性.
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