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相关概念视频

Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Global Regulatory Systems01:28

Global Regulatory Systems

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Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Cell Specific Gene Expression01:58

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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相关实验视频

Updated: Jul 17, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

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基因调控网络建模使用植物单细胞多基因组.

Tran Chau1, Prakash Timilsena2, Song Li3,4

  • 1Graduate Program in Genetics, Bioinformatics and Computational Biology (GBCB), Blacksburg, VA, USA.

Methods in molecular biology (Clifton, N.J.)
|September 8, 2023
PubMed
概括

这项研究提出了分析单细胞多组数据的计算管道,以建立植物中的基因调节网络. 这种方法有助于在单细胞水平上了解基因表达和染色质的可访问性.

关键词:
基因监管网络 基因监管网络机器学习是机器学习.动机分析 动机分析多个omics的多个omics.草根是一种根源.单细胞测序是一种单细胞测序.这就是 scATAC-seqq.这就是 scRNA-seqq.

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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

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相关实验视频

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科学领域:

  • 植物生物学 植物生物学
  • 基因组学就是基因组学.
  • 计算生物学是一种计算生物学.

背景情况:

  • 单细胞多组技术为细胞异质性提供了高分辨率的见解.
  • 了解基因调节对于破译复杂的生物过程至关重要.

研究的目的:

  • 描述用于分析单细胞RNA测序 (scRNA-seq) 和单细胞转移酶可访问染色体测序 (scATAC-seq) 数据的计算管道.
  • 从单细胞多组数据构建基因调节网络.

主要方法:

  • 数据规范化和 scRNA-seq 和 scATAC-seq 数据集的整合.
  • 集群标记基因的识别.
  • 为选择的标记基因寻找动机.
  • 机器学习应用程序用于监管网络识别.

主要成果:

  • 该管道成功地使用模型植物物种Arabidopsis.数据进行了验证.
  • 开发的管道可适应分析各种植物和动物物种的单细胞多组数据.

结论:

  • 计算管道提供了一个强大的框架,用于使用单细胞多omics数据来表征基因调节网络.
  • 这种方法有助于更深入地了解不同物种细胞水平的基因调节.