相关实验视频

Updated: Jul 17, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

基于针头开关的异热转录放大用于简单的微RNA灵敏度检测

Xi Long¹, Tong Luo¹, Panpan Yuan¹

¹College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, 410017 P. R. China.

Analytical chemistry

|September 8, 2023

概括

我们开发了一种用于敏感微RNA (miRNA) 检测的新型发针DNA开关. 该系统使生物样本中的miRNAs能够进行简单,选择性和敏感的量化.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
分析化学分析化学

背景情况:

在临床样本中检测低微RNA (miRNA) 水平至关重要,但具有挑战性.
现有的方法往往缺乏简单性,选择性或敏感性.

研究的目的:

开发一种新,高度敏感和选择性的miRNA检测系统.
为了克服当前miRNA量化技术的局限性.

主要方法:

设计了一种单链DNA发针结构,集成T7原料,模板,目标识别序列和RNA吸收体.
发针开关抑制T7转录,直到目标miRNA结合.
目标结合将发针展开,启动T7转录并产生用于信号放大的RNA吸收体.

主要成果:

该系统在miRNA检测方面表现出高的选择性和灵敏性.
在缓冲和复杂的生物样本中实现了miRNA的定量分析.
这种新的方法比现有的基于T7聚合酶的系统在简单性和方便性方面具有优势.

结论:

开发的针头开关系统为简单,选择性和敏感的miRNA检测提供了一个强大的平台.
这项技术在临床诊断和生物研究中具有潜在的应用.
该系统在复杂样品中的效率突出显示了其实用性.

更多相关视频

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

相关概念视频

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...