ATAC和SAGA联合激活器复合体利用共同翻译组合,但它们的细胞局部化特性和功能是不同的
Gizem Yayli1, Andrea Bernardini1, Paulina Karen Mendoza Sanchez2
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France; Université de Strasbourg, Illkirch, France.
Cell reports
|September 8, 2023
概括
ATAC和SAGA转录协激活体复合体以协译方式组装在一起. 虽然SAGA在细胞质中起作用,但ATAC在细胞核中运作明显,突出独特的组装和定位路径.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 了解多子单元复杂组合对于破译细胞功能至关重要.
- 同翻译组合,即蛋白质在翻译过程中相互作用,是关键机制.
- 像ATAC和SAGA这样的氨酸转移酶复合体在转录调节中起着至关重要的作用.
研究的目的:
- 为了研究ATAC (ADA-two-A-containing) 和SAGA (Spt-Ada-Gcn5-acetyltransferase) 复合物的组装机制.
- 为了确定ATAC和SAGA之间的亚细胞局部和功能差异.
- 阐明协译组合在这些转录协激活体复合体形成中的作用.
主要方法:
- 在哺乳动物细胞细胞质中对ATAC和SAGA核心模块的同翻译组合的分析.
- 检测SAGA复合体形成及其在细胞质中的乙转移酶活性.
- 亚细胞局部化研究,以确定ATAC和SAGA在不同细胞区中的存在和功能.
主要成果:
- ATAC和SAGA复合体都在哺乳动物细胞的细胞质中启动同翻译组合.
- 完整的SAGA复合体在细胞质中形成,并表现出非基因素蛋白乙化活性.
- 细胞质中没有检测到ATAC复合体子单元;相反,两个模块组成的功能性ATAC复合体在核中形成并运作.
结论:
- ATAC和SAGA虽然相关,但表现出不同的亚细胞局部,细胞质丰度和功能作用.
- 同翻译组合是两种复合体的保留途径,但它们随后的细胞内运输和功能显著分离.
- 这项研究揭示了相关的转录辅激剂复合体的差异组装和局部化策略.
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