对莉花受体的遗传分析揭示了OsCOI2的特殊功能
Hieu Trang Nguyen1, Mohamad Cheaib1, Marie Fournel1,2
1DIADE, IRD, Univ Montpellier, Montpellier, France.
PloS one
|September 8, 2023
概括
米中雅斯蒙酸盐的感知涉及OsCOI2,这是一个非正规的受体,对根,叶和花的发育至关重要. 这项研究揭示了OsCOI2
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 植物生理学 植物生理学
背景情况:
- 莉花酸的信号传递对植物发育和应激反应至关重要.
- 米 (单种) 具有两个COI基因组 (OsCOI1a/b),类似于双种COI1,以及一个未表征的OsCOI2.
- OsCOI2的特定作用及其与大米中的OsCOI1基因的关系在很大程度上是未知的.
研究的目的:
- 为了阐明大米OsCOI2基因的功能.
- 为了研究OsCOI1a,OsCOI1b和OsCOI2.2之间的功能冗余.
- 鉴定米中涉及这些COI基因的莉花反应途径.
主要方法:
- 通过CRISPR Cas9基因编辑,制造了OsCOI1a,OsCOI1b和OsCOI2的突变.
- 生成的突变体的表型特征.
- 对莉花治疗的突变反应的评估.
主要成果:
- OsCOI2在根,叶子和花朵的发育中起着重要的作用.
- 雅斯莫纳酸诱导的皇冠根生长抑制取决于OsCOI2,而不是OsCOI1a或OsCOI1b.
- OsCOI2在莉花酸中介的根部发育中表现出一种专门的,非正规的功能.
结论:
- OsCOI2在调节大米植物发育方面有着独特而重要的作用.
- 贾斯蒙酸盐受体的亚功能化已经发生在单子系内.
- 这项研究强调了OsCOI2在米中莉花酸信号通路中的特殊功能.
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