类固醇受体联合激活剂TAIMAN是一种昆虫昼夜时钟的新调节器
Vlastimil Smykal1, Lenka Chodakova1,2, Marketa Hejnikova1
1Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Entomology, Ceske Budejovice, Czech Republic.
PLoS genetics
|September 8, 2023
概括
泰曼 (TAI) 是昆虫发育的关键调节者. 这项研究揭示了TAI.
科学领域:
- 昆虫内分泌学 昆虫内分泌学
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 泰曼 (TAI) 是哺乳动物类固醇受体联合激活剂 (SRCs) 的唯一昆虫 Ortholog.
- TAI通过青春激素 (JH) 和ecdysone信号通路调节昆虫的发育和繁殖.
- 昆虫激素与环境线索相互作用,如光周期,以调节生理学.
研究的目的:
- 调查JH和ecdysone信号在昆虫昼夜钟中的作用.
- 为了确定TAIMAN (TAI) 是否影响昆虫的昼夜节律.
- 探索TAI在昆虫物种中昼夜钟调节中的保存功能.
主要方法:
- 在Pyrrhocoris apterus和Blattella germanica中使用双链RNA对泰曼的基因敲除.
- 微手术和激素/模仿剂的应用,以研究信号通路.
- 生物钟自由运行周期 (FRP) 的测量.
主要成果:
- 沉默泰曼显著减缓了白虫和的昼夜时钟的自由运行周期 (FRP).
- 观察到的时钟减速效应在远距离相关的昆虫物种中得到保护.
- 阻断或补充JH和ecdysone信号,或击倒它们的受体,不会影响昼夜时钟节奏.
结论:
- 泰曼 (TAI) 作为昆虫昼夜时钟的新型调节器.
- 这代表了TAI在昆虫中的时钟调节作用的首次描述.
- 在生物钟中TAI的功能可能在动物中得到保护,类似于哺乳动物SRC-2在新陈代谢和节律中的作用.
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