在Arabidopsis中对lncRNAs的种群级注释揭示了与可转移元素类沉默相关的广泛表达变异
Aleksandra E Kornienko1, Viktoria Nizhynska1, Almudena Molla Morales1
1Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-gasse 3, Vienna 1030, Austria.
The Plant cell
|September 8, 2023
概括
研究人员绘制了数千个植物长非编码RNA (lncRNA) 位点的地图,发现大多数是沉默的,并且由于染色质变化而表现出高度的表达变异性,特别是在与可转移元素相关的跨基因lncRNA中.
科学领域:
- 植物基因组学 植物基因组学
- 文字转录学 (Transcriptomics) 是一个学科.
- 分子生物学分子生物学
背景情况:
- 长非编码RNAs (lncRNAs) 是关键的调节分子,但它们在植物中的注释和表达模式与哺乳动物相比仍然基本未被探索.
- 已知 lncRNA 表达在物种内具有很高的变异性,这表明它在自然变异中起着重要的作用.
研究的目的:
- 为了全面注释 lncRNA 位点在广泛的自然 Arabidopsis thaliana 加入.
- 研究植物中lncRNA转录的自然变异程度及其潜在的调节机制.
主要方法:
- 利用了来自数百个自然Arabidopsis加入和多个发育阶段的深度转录组测序数据.
- 开发了 lncRNA loci. 的全人口注释.
- 分析了lncRNA表达,染色质状态和可转移元素 (TE) 含量之间的关系.
主要成果:
- 鉴定了数千个以前未被注释的lncRNA位点,表明植物基因组中无处不在的转录.
- 观察到,大多数lncRNA位点被积极沉默,在自然加入中表达高度变化.
- 发现抑制色素变异性是 lncRNA 表达变化的主要驱动因素,特别是含有可转移元素的跨基因 lncRNA (lincRNA).
结论:
- 这项研究为Arabidopsis提供了基础的全人口lncRNA注释,大大提高了我们对植物lncRNA基因组生物学的理解.
- 这些发现突出了由染色质修饰和TE相互作用驱动的lncRNA转录和沉默的动态调节,引发了关于植物基因组调节的新问题.
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